Detection of Orally Administered

Chromatographic analysis of amantadine was achieved using an Agilent J&W CP-Sil 8 CB column. Published by Elsevier B. V. Spatial Reorganization of Saccharomyces cerevisiae Enolase To Alter Carbon Metabolism under Hypoxia Eukaryotic Cell, 12, 1106-1119 (2013) Natsuko Miura et al. Tags CP-Sil 8 CB, clinical research Abstract Derivatized metabolites in a study of yeast hypoxia were analyzed by GC/MS with an Agilent J&W CP-Sil 8 CB column. Published by the American Society for Microbiology.Derivatized metabolites in a study of yeast hypoxia were analyzed by GC/MS with an Agilent J&W CP-Sil 8 CB column. Published by the American Society for Microbiology. Metabolic Profiling of Oxidized Lipid-Derived Volatiles in Blood by Gas Chromatography/Mass Spectrometry with In-Tube Extraction Mass Spectrometry (Tokyo), 10 (2013) Shoji Kakuta et al. Tags CP-Sil 8 CB, clinical research Abstract The authors applied a GC/MS method using an Agilent J&W GC column to mouse plasma and identified 12 volatiles, including 1-octen-3-ol, which is correlated to disease states. Published by the Mass Spectrometry Society of Japan.The authors applied a GC/MS method using an Agilent J&W GC column to mouse plasma and identified 12 volatiles, including 1-octen-3-ol, which is correlated to disease states. Published by the Mass Spectrometry Society of Japan. Detection of Orally Administered Inositol Stereoisomers in Mouse Blood Plasma and Their Effects on Translocation of Glucose Transporter 4 in Skeletal Muscle Cells Journal of Agricultural and Food Chemistry, 61, 4850–4854 (2013) Yoko Yamashita et al. Tags CP-Sil 8 CB, 7890A GC, 7683B Autosampler, clinical research Abstract Simple pharmacological studies on inositol stereoisomers are presented in this study. Male ICR mice were orally administered 1 g/kg BW of three inositol stereoisomers, myo-inositol (MI), dchiro-inositol (DCI), and scyllo-inositol (SI), and blood plasma samples and skeletal muscle fractions were prepared after an hour. The plasma samples were subjected to gas chromatography–coupled time-of-flight mass spectrometry (GC-TOF-MS) analysis. None of the three stereoisomers was seen in untreated samples, but substantial amounts ranging from 2.5 to 6.5 mM were detected only after administration, indicating that orally administered inositol stereoisomers were readily absorbed and their levels elevated in the bloodstream. In addition, plasma of SI-administered animals contained substantial MI, suggesting a possible metabolic conversion of SI to MI. In the skeletal muscle fractions, glucose transporter type 4 (GLUT4) content in the plasma membrane increased, indicating that inositol stereoisomers stimulated GLUT4 translocation. Reprinted with permission from the Journal of Agricultural and Food Chemistry. Copyright 2013 American Chemical Society.Simple pharmacological studies on inositol stereoisomers are presented in this study. Male ICR mice were orally administered 1 g/kg BW of three inositol stereoisomers, myo-inositol (MI), dchiro-inositol (DCI), and scyllo-inositol (SI), and blood plasma samples and skeletal muscle fractions were prepared after an hour. The plasma samples were subjected to gas chromatography–coupled time-of-flight mass spectrometry (GC-TOF-MS) analysis. None of the three stereoisomers was seen in untreated samples, but substantial amounts ranging from 2.5 to 6.5 mM were detected only after administration, indicating that orally administered inositol stereoisomers were readily absorbed and their levels elevated in the bloodstream. In addition, plasma of SI-administered animals contained substantial MI, suggesting a possible metabolic conversion of SI to MI. In the skeletal muscle fractions, glucose transporter type 4 (GLUT4) content in the plasma membrane increased, indicating that inositol stereoisomers stimulated GLUT4 translocation. Reprinted with permission from the Journal of Agricultural and Food Chemistry. Copyright 2013 American Chemical Society. A novel serum metabolomics-based diagnostic approach to pancreatic cancer Cancer Epidemiology, Biomarkers and Prevention, 22, 571-579 (2013) Takashi Kobayashi et al. Tags CP-Sil 8 CB, clinical research Abstract In an assessment of tumor markers for pancreatic cancer, serum metabolites were analyzed by GC/MS with an Agilent J&W CP-Sil 8 CB column. Published by the American Association for Cancer Research.In an assessment of tumor markers for pancreatic cancer, serum metabolites were analyzed by GC/MS with an Agilent J&W CP-Sil 8 CB column. Published by the American Association for Cancer Research. Metabolic profiling of human follicular fluid identifies potential biomarkers of oocyte developmental competence Reproduction,146, 389–395 (2013) A. O'Gorman et al. Tags CP-Sil 8 CB, 7890A GC, 5975C MS, ChemStation, clinical research Abstract The use of metabolomic based techniques to aid oocyte and embryo selection has gained attention in recent years. Previous work from our laboratory has demonstrated that the 1H NMR-based metabolic profile of follicular fluid correlates with oocyte developmental potential. Patients undergoing IVF at the Merrion Fertility Clinic had follicular fluid collected at the time of oocyte retrieval. The fatty acid composition of follicular fluid from follicles where oocytes fertilised and developed into multi-cell embryos (n=15) and from oocytes that fertilised normally but failed to cleave (n=9) (cleaved vs non-cleaved) was compared. Statistical analysis was performed on the data using univariate and multivariate techniques. Analysis of the fatty acid composition revealed that there were nine fatty acids significantly different between follicular fluid from the cleaved and the non-cleaved sample groups. Of particular interest were the higher concentration of total saturated (P=0.03) and the lower concentration of total polyunsaturated fatty acids in the noncleaved sample group (P=0.001). Random forest classification models were used to predict successful cleavage in follicular fluid samples producing models with errors rates of <10%. Receiver operating characteristic analysis demonstrated that the model had good predictability with an area under the curve of 0.96. The panel of fatty acid biomarkers identified in this study indicates that the fatty acid composition of follicular fluid may be more predictive in comparison to other previously identified biomarkers. Following validation in a larger cohort, these biomarkers may have the potential to be used in fertility clinics to aid the selection of oocytes in the future. Published by the Society for Reproduction and Fertility.The use of metabolomic based techniques to aid oocyte and embryo selection has gained attention in recent years. Previous work from our laboratory has demonstrated that the 1H NMR-based metabolic profile of follicular fluid correlates with oocyte developmental potential. Patients undergoing IVF at the Merrion Fertility Clinic had follicular fluid collected at the time of oocyte retrieval. The fatty acid composition of follicular fluid from follicles where oocytes fertilised and developed into multi-cell embryos (n=15) and from oocytes that fertilised normally but failed to cleave (n=9) (cleaved vs non-cleaved) was compared. Statistical analysis was performed on the data using univariate and multivariate techniques. Analysis of the fatty acid composition revealed that there were nine fatty acids significantly different between follicular fluid from the cleaved and the non-cleaved sample groups. Of particular interest were the higher concentration of total saturated (P=0.03) and the lower concentration of total polyunsaturated fatty acids in the noncleaved sample group (P=0.001). Random forest classification models were used to predict successful cleavage in follicular fluid samples producing models with errors rates of <10%. Receiver operating characteristic analysis demonstrated that the model had good predictability with an area under the curve of 0.96. The panel of fatty acid biomarkers identified in this study indicates that the fatty acid composition of follicular fluid may be more predictive in comparison to other previously identified biomarkers. Following validation in a larger cohort, these biomarkers may have the potential to be used in fertility clinics to aid the selection of oocytes in the future. Published by the Society for Reproduction and Fertility. Serum and Tissue Metabolomics of Head and Neck Cancer Cancer Genomics and Proteomics, 10, 233-238 (2013) Koichiro Yonezawa et al. Tags CP-Sil 8 CB, clinical research Abstract The metabolomic analysis of serum and tissue samples obtained from patients with head and neck cancer was performed using GC/MS and an Agilent J&W CP-Sil 8 CB column. Published by the International Institute of Anticancer Research.The metabolomic analysis of serum and tissue samples obtained from patients with head and neck cancer was performed using GC/MS and an Agilent J&W CP-Sil 8 CB column. Published by the International Institute of Anticancer Research. Local administration of growth hormone stimulates Journal of Applied Physiology, 113, 1432-1438 (2012) Poul Vestergaard et al. Tags CP-Sil 8 CB, CP-Sil 19 CB, clinical research Abstract Plasma for determination of phenylalanine enrichment was prepared and analyzed as its tbutyldimethylsilyl derivative by GC/MS and separated by an Agilent CP-Sil 8 CB GC column. Amino acids were derivatized as their N-acetyl-n-propyl esters and the 13C abundance under the phenylalanine peak was determined by GC/combustion isotope ratio MS with an Agilent J&W CP-Sil 19 CB column. Published by the American Physiological Society.Plasma for determination of phenylalanine enrichment was prepared and analyzed as its tbutyldimethylsilyl derivative by GC/MS and separated by an Agilent CP-Sil 8 CB GC column. Amino acids were derivatized as their N-acetyl-n-propyl esters and the 13C abundance under the phenylalanine peak was determined by GC/combustion isotope ratio MS with an Agilent J&W CP-Sil 19 CB column. Published by the American Physiological Society. Energy and chemicals Capillary GC column performance test for basic analytes Journal of Microcolumn Separations, 10, 661– 669 (1998) R. J. M. N. Dresen, H. J. W. Henderickx, Sj. van der Wal Tags CAM, CP-Sil 8 CB for Amines, energy and chemicals Abstract An investigation of GC columns for basic analytes found that Agilent J&W CP-Sil 8 CB for Amines and CAM columns were best suited for analysis of amines at low concentration levels with respect to irreversible adsorption. Published by John Wiley and Sons.An investigation of GC columns for basic analytes found that Agilent J&W CP-Sil 8 CB for Amines and CAM columns were best suited for analysis of amines at low concentration levels with respect to irreversible adsorption. Published by John Wiley and Sons. Copper (II) aza-bis (oxazoline) complex immobilized onto ITQ-2 and MCM-22 based materials as heterogeneous catalysts for the cyclopropanation of styrene Microporous and Mesoporous Materials, 179, 231-241 (2013) Ana R. Silva et al. Tags CP-Sil 8 CB, CP-Chirasil-Dex CB, energy and chemicals, biofuels and alternative energy Abstract Reaction mixture was analyzed by GC-FID on a gas chromatograph equipped with a fused silica Agilent J&W CP-Sil 8 CB column. Enantiomeric excesses of the cyclopropanes were determined in the same chromatograph but using an Agilent J&W CP-Chirasil-Dex CB column. Published by Elsevier B. V.Reaction mixture was analyzed by GC-FID on a gas chromatograph equipped with a fused silica Agilent J&W CP-Sil 8 CB column. Enantiomeric excesses of the cyclopropanes were determined in the same chromatograph but using an Agilent J&W CP-Chirasil-Dex CB column. Published by Elsevier B. V. Simultaneous derivatization and air-assisted liquid–liquid microextraction of some aliphatic amines in different aqueous samples followed by gas chromatography-flame ionization detection Analytica Chimica Acta, 775, 50-57 (2013) Mir Ali Farajzadeh, Nina Nouri Tags CP-Sil 8 CB, energy and chemicals Abstract Separation and determination of selected aliphatic amines was accomplished using an Agilent J&W CP-Sil 8 CB GC column. Published by Elsevier.Separation and determination of selected aliphatic amines was accomplished using an Agilent J&W CP-Sil 8 CB GC column. Published by Elsevier. Dynamic capillary diffusion system for monoterpene and sesquiterpene calibration: quantitative measurement and determination of physical properties International Journal of Environmental Analytical Chemistry, 93, 637-649 (2013) Jiri Kosina et al. Tags CP-Sil 8 CB, VF-1ms, energy and chemicals Abstract This article describes a method for preparation of low concentration gas standard mixtures of biogenic volatile organic compounds (BVOCs) emitted primarily by plants. A set of 10 plant volatiles including α-pinene, β-pinene, 3-carene, linalool, methyl salicylate, α-cedrene, β-caryophyllene, βfarnesene, aromadendrene and α-humulene was used in the study. Gas standard mixture of these compounds was generated using a capillary diffusion system (CDS). Diffusion coefficient (D) and saturation vapour pressure (p s) data of these compounds were calculated from experimentally determined gas chromatographic retention indices (RI) and empirical relationships between D and p s versus RI. A comparison of the calculated and measured concentrations of investigated compounds has proved that designed CDS can be successfully used for the proper quantification of BVOCs. © 2013 Taylor and Francis.This article describes a method for preparation of low concentration gas standard mixtures of biogenic volatile organic compounds (BVOCs) emitted primarily by plants. A set of 10 plant volatiles including α-pinene, β-pinene, 3-carene, linalool, methyl salicylate, α-cedrene, β-caryophyllene, βfarnesene, aromadendrene and α-humulene was used in the study. Gas standard mixture of these compounds was generated using a capillary diffusion system (CDS). Diffusion coefficient (D) and saturation vapour pressure (p s) data of these compounds were calculated from experimentally determined gas chromatographic retention indices (RI) and empirical relationships between D and p s versus RI. A comparison of the calculated and measured concentrations of investigated compounds has proved that designed CDS can be successfully used for the proper quantification of BVOCs. © 2013 Taylor and Francis. Heterogeneous catalytic epoxidation of olefin over a hydrothermally synthesized 3D phosphate bridged copper (II) framework Journal of Coordination Chemistry, 66, 24442454 (2013) D. Saha et al. Tags CP-Sil 8 CB, energy and chemicals Abstract A 3D copper phosphate, [Cu2(PO4)(OH)] n (1), has been synthesized hydrothermally and characterized by single-crystal X-ray diffraction analysis. In [Cu2(PO4)(OH)] n , there are two types of copper centers having distorted trigonal bipyramidal geometry and distorted octahedral geometry that are connected by the μ2-bridging of each phosphate oxygen ultimately forming a η-PO4 bridged 3D network. The compound exhibited excellent catalytic performance in olefin epoxidation. Epoxidation of styrene and substituted styrenes, as well as bulky olefins like cycloalkenes and long-chain alkenes, is efficiently catalyzed by [Cu2(PO4)(OH)] n using tert-butylhydroperoxide in acetonitrile. The results obtained in the heterogeneous catalytic reactions show that the olefins are converted to the respective epoxides in good yield with high selectivity. [Cu2(PO4)(OH)] n was catalytically more active and selective in comparison to simple copper(II) phosphate salt in heterogeneous medium. The catalyst can be recycled and reused several times without significant loss of activity. © 2013 Taylor and Francis.A 3D copper phosphate, [Cu2(PO4)(OH)] n (1), has been synthesized hydrothermally and characterized by single-crystal X-ray diffraction analysis. In [Cu2(PO4)(OH)] n , there are two types of copper centers having distorted trigonal bipyramidal geometry and distorted octahedral geometry that are connected by the μ2-bridging of each phosphate oxygen ultimately forming a η-PO4 bridged 3D network. The compound exhibited excellent catalytic performance in olefin epoxidation. Epoxidation of styrene and substituted styrenes, as well as bulky olefins like cycloalkenes and long-chain alkenes, is efficiently catalyzed by [Cu2(PO4)(OH)] n using tert-butylhydroperoxide in acetonitrile. The results obtained in the heterogeneous catalytic reactions show that the olefins are converted to the respective epoxides in good yield with high selectivity. [Cu2(PO4)(OH)] n was catalytically more active and selective in comparison to simple copper(II) phosphate salt in heterogeneous medium. The catalyst can be recycled and reused several times without significant loss of activity. © 2013 Taylor and Francis. Hydrogen Production from Methanol Steam Reforming over Cu/ZnO/Al O/CeO/ZrO Nanocatalyst in an Adiabatic Fixed-Bed Reactor Iranica Journal of Energy & Environment, 3, 307313 (2012) Hassan Sharifi Pajaie, Majid Taghizadeh, Ali Eliassi Tags CP-Sil 8 CB, energy and chemicals Abstract Hydrogen production from steam reforming of methanol was performed in an adiabatic fixed bed heterogeneous reactor by using Cu/ZnO/Al2O3/CeO2 /ZrO2 nanocatalyst. The catalyst was prepared by co-precipitation method and characterized by TGA, XRD, SEM and BET. By changing the mean average temperature of the catalyst bed (or reactor operation temperature) from 230 to 270°C, variations in methanol conversion were monitored. The results showed that the conversion of methanol was strongly dependent on the reaction temperature. In addition, the effects of weight hourly space velocity (WHSV) on methanol conversion were investigated. According to the results, the maximum conversion was obtained at 270°C with WHSV of 15 h 1.Hydrogen production from steam reforming of methanol was performed in an adiabatic fixed bed heterogeneous reactor by using Cu/ZnO/Al2O3/CeO2 /ZrO2 nanocatalyst. The catalyst was prepared by co-precipitation method and characterized by TGA, XRD, SEM and BET. By changing the mean average temperature of the catalyst bed (or reactor operation temperature) from 230 to 270°C, variations in methanol conversion were monitored. The results showed that the conversion of methanol was strongly dependent on the reaction temperature. In addition, the effects of weight hourly space velocity (WHSV) on methanol conversion were investigated. According to the results, the maximum conversion was obtained at 270°C with WHSV of 15 h 1. Kinetics of Degradation of perchloroethylene under Ultrasonic Irradiation and Photooxidation in Aqueous Solution Environmental Protection Engineering, 39, 29-38 (2013) Mahdi Kargar et al. Tags CP-Sil 8 CB, energy and chemicals Abstract Perchloroethylene (PCE) is mainly used as a solvent and degreaser. In this work, PES was analyzed by head space gas chromatography with FID detection, using an Agilent J&W CP-Sil 8 CB column.Perchloroethylene (PCE) is mainly used as a solvent and degreaser. In this work, PES was analyzed by head space gas chromatography with FID detection, using an Agilent J&W CP-Sil 8 CB column.